CENTER OF EXCELLENCE FOR MOLECULAR FOOD SCIENCES

Home page

Events

Documents

Equipment

Spectrofluorimeter FluoroMax-4, HORIBA, Jobin Yvon

Spectrofluorimeter FluoroMax-4 HORIBA Jobin Yvon offers analytical speed and the ultimate sensitivity in fluorescence investigations. Fluorescence spectroscopy has assumed a major role in analysis, as for applicable compounds fluorescence gives high sensitivity and high specificity. High sensitivity results from a difference in wavelength between the exciting and fluorescence radiation. These results in a signal contrasted with essentially zero background. High specificity results from dependence on two spectra: the excitation and the emission spectrum. 3-D matrix scanning and contour-mapping provide a unique fingerprint that qualitatively identifies a compound. In florescence analysis, the amount of light emitted characteristically under suitable excitation is used as a measure of the concentration of the responsible species. Concentrations as low as fg/ml can be determined.

Applications:

1. Estimation of subtle differences in protein tertiary structure by monitoring intrinsic tryptophane fluorescence of proteins.
2. Determination of binding constants of small molecules (such as polyphenols) to proteins by tryptophan fluorescence quenching method.
3. Investigation of protein surface hydrophobicity (or small changes in tertiary structure influencing surface hydrophobicity) by monitoring of binding of hydrophobic fluorescent probe (such as ANS, 8-anilino-1-naphtalensulfonic acid).
4. Monitoring of Maillard reaction by detection of fluorescent Maillard reaction products.
5. Highly sensitive determination of enzyme activity with fluorescent substrate.
6. Evaluation of interactions of biologically active coordinated compounds, such as platinum (IV) and ruthenium (II) complexes, with human serum albumin and calf thymus DNA.
7. Correlation of structure and features of natural and synthetic molecules and their metal complexes.

Contact person:

Prof. dr Ljuba Mandić
lab. 403
phone: (+381 11) 333 66 76
device is located in room 509

Characteristics:

1. Excitation: Range 200-950nm
2. Emission: Range 200-950nm
3. Bandpass: 0-15nm, continuously adjustable from computer
4. Integration time: From 1 ms to 160 s
5. Scan speed: 80 nm/s
6. Water Raman signal: min. 400.000 cps minimum at 350 nm excitation, 397 nm emission, 5 nm bandpass, 1 s integration time
7. Signal/Noise ratio: 3000/1
8. Thermostated single cuvette holder. Designed for 1 cm pathlength cuvettes. Maintains temperature from -20° to 80°C.