Molecular Genetics   (064B2)

Academic year:

2024/2025.

Attendance requirements:

062B2

ECTS:

6

Study level:

Study program:

Teacher:

Milica P. Keckarević-Marković, Ph.D.
assistant professor, Faculty of Biology, Studentski trg 3, Beograd

Assistant:

Milica Lj. Mihajlović Srejić

Hours of instruction:

Weekly: three hours of lectures + two hours of labwork (3+0+2)

Goals:

The goal of this course is to introduce students to the fundamentals of molecular genetics, particularly to the phenomena regarding the genome expression (recombination and the mechanisms of the regulation of gene expression); to the basic methods and model systems in molecular genetics; to new scientific disciplines such as genomics and comparative genomics; to the application of basic knowledge of molecular genetics in the study of complex cellular processes, such as the cell death of the apoptosis type and neoplastic transformation.

Outcome:

Students have mastered the fundamentals of molecular genetics, particularly the phenomena regarding the genome expression (recombination and the mechanisms of the regulation of gene expression). They have also mastered the basic methods and model systems in molecular genetics.

Teaching methods:

Lectures, theory exercises.

Extracurricular activities:

—

Coursebooks:

Main coursebooks:

• Goran Brajušković: Molekularna biologija 2. Beograd, Savremena administracija 2012.

Supplementary coursebooks:

• J. D. Watson, T. A. Baker, S. P. Bell, A. Gann, M. Levine, R. Losick (editors): Molecular Biology of the Gene. 5^th Edition, San Francisco, Pearson Education Inc. 2004

Additional material:

—

Lectures:

0 points (3 hours a week)

Syllabus:

1. Historical perspectives of molecular genetics
2. Homologous recombination
3. Site-specific recombination
4. Transposition
5. The regulation of gene expression in prokaryotes
6. The regulation of gene expression in eukaryotes
7. Regulatory RNAs in prokaryotes
8. Regulatory RNAs in eukaryotes
9. RNA interference
10. DNA methylation and genomic imprinting
11. The molecular biological methods for DNA analysis
12. The molecular biological methods for RNA analysis
13. The molecular biological methods for protein analysis
14. Model systems in molecular genetics

Labwork:

0 points (2 hours a week)

Syllabus:

1. Molecular biological methods for the analysis of chromosomes
2. Molecular biological methods for the analysis of DNA molecules
3. Molecular biological methods for the analysis of RNA molecules
4. Polymerase chain reaction (PCR), RT-PCR and "real-time" PCR
5. Molecular biological methods for the analysis of proteins
6. Molecular biological methods for mutation detection
7. Molecular biological methods for mutation screening
8. Array methods
9. Molecular biological methods for genome sequencing
10. Methods used for sequencing large genomes
11. The defense of students' term papers
12. The defense of students' term papers
13. The defense of students' term papers
14. A visit to a laboratory of molecular biology
15. A visit to a laboratory of molecular biology

Colloquia:

60 points

Remarks:

There are three progress tests altogether, each test carries 20 points.

Oral exam:

40 points