Course code:
1312A		 Course name:
Separation Methods

Academic year:

2012/2013.

Attendance requirements:

1302H / 1302P / 1301B

ECTS:

6

Study level:

basic academic studies

Study programs:

Chemistry: 3. year, summer semester, elective (1IP3A) course

Biochemistry: 3. year, summer semester, elective (1IP3B) course

Chemistry Teacher: 3. year, summer semester, elective (1IP3A) course

Teacher:

Rada M. Baošić, Ph.D.
full professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Assistants:

—

Hours of instruction:

Weekly: four hours of lectures (4+0+0)

Goals:

Student is during the course introduces with the basic principles of separation methods and ways of selecting the appropriate method in relation to the requirements.

Outcome:

A student is qualified to make the selection and application of the most suitable method for the appropriate separation.

Teaching methods:

Lectures and seminar works.

Extracurricular activities:

—

Coursebooks:

Main coursebooks:

  • C. E. Meloan, Chemical Separation, principles, techniques, and experiments, John Wiley & Sons inc, New York, 1999.  
  • S. Ahuja, Handbook of bioseparations, Academic press, California, 2000.

Supplementary coursebooks:

  • D. Harvey, Modern Analytical Chemistry, McGraw-Hill, Boston, 2000.
  • D. C. Harris, Quantitative Chemical Analysis, Fifth Edition, W.H.Freeman and Company, New York, 1999.
  • M. Radojevic, V. Baskhin, Practical environmental analysis, The Royal Society of Chemistry, Cambridge, UK, 1999.

Additional material:

—

  Course activities and grading method

Lectures:

10 points (4 hours a week)

Syllabus:

Sample preparation. Extraction. Liquid-liquid, liquid-solid and solid-liquid extraction. Solid phase extraction. Supercritical extraction. Head-space technique. Ionic exchange. Separation methods based on different principles of chromatography. Optimisation of chromatographic separation. Electrophoresis. Capillary elecrophoresis. Isoelectric focusing. Dialisys.  Applications of mass spectrometry in analysis of biomolecules. Effect of applied detectors on efficiency of separation method. Selection of appropriate separation methods for the quantitative analysis of various real samples.

Semester papers:

40 points

Oral exam:

50 points