Calculations in Biochemistry and Molecular Biology   (405B2)

Academic year:

2023/2024.

Attendance requirements:

There are no requirements.

ECTS:

5

Study level:

Study program:

Teacher:

Marija M. Stojadinović, Ph.D.
assistant professor, Faculty of Chemistry, Studentski trg 12-16, Beograd

Assistant:

Mirjana Ž. Radomirović
associate researcher, Faculty of Chemistry, Studentski trg 12-16, Beograd

Hours of instruction:

Weekly: two hours of lectures + one hour of exercises (2+1+0)

Goals:

The goal of this course is to help students master the basic calculations in biochemistry, molecular biology and cell biology.

Outcome:

Students are completely able to do basic calculations in biochemistry molecular biology and cell biology.

Teaching methods:

Practical classes.

Extracurricular activities:

—

Coursebooks:

• Lisa A. Sedman: Basic Laboratory Calculations for Biotechnology
• Frank H. Stephenoson: Calculations for Molecular Biology and Biotechnology

Additional material:

—

Lectures:

0 points (2 hours a week)

Syllabus:

1. Preparation of solutions in biochemistry. Buffers. Media.
2. Introduction to statistical data processing.
3. Basic calculations in cell biology; quantity and concentration of nucleic acids.
4. Basic calculations in molecular biology: restriction digests, the analysis of fragments on agarose gel; the transformation efficiency; determination of molecular weight of DNA, RNA and proteins.
5. The PCR reaction: components and setting up a PCR reaction; Enzymes, nucleotides, primers, the matrix.
6. Basic calculations in biochemistry: determination of total and specific proteins; the purification factor and quantity; determination of specific proteins: enzyme activity.
7. Working with bacteriophages.
8. Labeling nucleic acids with radioisotopes.
9. Centrifuging. Calculating sedimentation time.

Exercises:

20 points (1 hour a week)

Colloquia:

40 points

Written exam:

40 points