Opis istraživanja
RAZVOJ I PRIMENA "OMICS" TEHNOLOGIJA I BIOLOŠKE
MASENE SPEKTROMETRIJE
U OBLASTIMA HRANE, POŠOPRIVREDE I MEDICINE
Metodama "shotgun" proteomike i imunoproteomike se otkrivaju
antigeni za dizajn vakcina za lečenje infekcija izazvanih intracelularnim
bakterijama poput hlamidije, ali i za ispitivanje posttranslacionih
modifikacija proteina i bolje razumevanje biologije ovih organizama i
infekcija koje izazivaju [1]. Modifikacijama antigena može da se poboljša
efikasnost i bezbednost vakcina. Novi proteinski terapeutici se testiraju
in vitro i in vivo metodama [2, 3].
Posebno se proučava kako struktura i modifikacije proteina
utiču na dinamiku degradacije proteina u endolizozomalnom odeljku a time i
na tip imunog odgovora koji dati protein indukuje [4].
Modifikacije proteina izazvane obradom hrane mogu da utiču
na procese digestije i preuzimanje proteina hrane u gastrointestinalnom
sistemu, kao i na procese koje ovi proteini indukuju u ćelijama imunog
sistema [5-7].
Metode protoemike i transkriptomike se kombinuju u cilju
identifikacije i karakterizacije novih uzročnika alergija, unapređenja
dijagnostike alergija i proizvodnje rekombinantnih alergena [8-10].
Metodama masene spektrometrije ispituje se polifenolni
sastav različitih biljnih tkiva [11]. Mehanizam dejstva polifenola u
imunomodulaciji se testira biohemijskim i metodama ćelijske biologije [12].
Metodama peptidomike se određuje struktura peptida nastalih dejstvom enzima
digestivnog trakta [13, 14]. Nastali peptidi se izoluju u cilju
karakterizacije biološke aktivnosti [15].
Interakcije biološki značajnih molekula hrane i proteina se
proučavaju eksperimentalnim i teorijskim metodama. Određuje se afinitet
vezivanja, tip interakcija uključenih u proces vezivanja, broj vezivnih mesta,
efekti vezivanja na strukturu i stabilnost proteina i liganda [16-22].
Odabrane reference:
- Mihailovic J, Inic-Kanada A, Smiljanic K, Stein E, Barisani-Asenbauer
T, Cirkovic Velickovic T: Lysine acetylation of major Chlamydia
trachomatis antigens. EuPA Open Proteomics 2016,
10:63-69.
- Stanic-Vucinic D, Stojadinovic M, Mirkov I, Apostolovic D, Burazer L,
Atanaskovic-Markovic M, Kataranovski M, Cirkovic Velickovic T:
Hypoallergenic acid-sensitive modification preserves major mugwort
allergen fold and delivers full repertoire of MHC class II-binding peptides
during endolysosomal degradation. RSC Advances 2016,
6(91):88216-88228.
- Apostolovic D, Luykx D, Warmenhoven H, Verbart D, Stanic-Vucinic D, De
Jong GAH, Velickovic TC, Koppelman SJ: Reduction and alkylation of
peanut allergen isoforms Ara h 2 and Ara h 6; Characterization of
intermediate- and end products. Biochimica et Biophysica Acta
- Proteins and Proteomics 2013,
1834(12):2832-2842.
- Stojadinovic M, Pieters R, Smit J, Velickovic TC: Cross-linking
of β-lactoglobulin enhances allergic sensitization through changes in
cellular uptake and processing. Toxicological Sciences
2014, 140(1):224-235.
- Mihajlovic L, Radosavljevic J, Nordlund E, Krstic M, Bohn T, Smit J,
Buchert J, Cirkovic Velickovic T: Peanut protein structure,
polyphenol content and immune response to peanut proteins: In vivo are
modulated by laccase. Food and Function 2016,
7(5):2357-2366.
- Radosavljevic J, Nordlund E, Mihajlovic L, Krstic M, Bohn T, Buchert J,
Velickovic TC, Smit J: Sensitizing potential of enzymatically
cross-linked peanut proteins in a mouse model of peanut allergy.
Molecular Nutrition and Food Research 2014,
58(3):635-646.
- Perusko M, Al-Hanish A, Velickovic TC, Stanic-Vucinic D:
Macromolecular crowding conditions enhance glycation and oxidation
of whey proteins in ultrasound-induced Maillard reaction. Food
Chemistry 2015, 177:248-257.
- Apostolovic D, Tran TAT, Hamsten C, Starkhammar M, Cirkovic Velickovic
T, Van Hage M: Immunoproteomics of processed beef proteins reveal
novel galactose-α-1,3-galactose-containing allergens.
Allergy: European Journal of Allergy and Clinical Immunology 2014,
69(10):1308-1315.
- Smiljanic K, Apostolovic D, Trifunovic S, Ognjenovic J, Perusko M,
Mihajlovic L, Burazer L, van Hage M, Cirkovic Velickovic T:
Subpollen particles are rich carriers of major short ragweed
allergens and NADH dehydrogenases: Quantitative proteomic and allergomic
study. Clinical and Experimental Allergy 2017.
- Apostolovic D, Tran TAT, Ssnchez-Vidaurre S, Cirkovic Velickovic T,
Starkhammar M, Hamsten C, Van Hage M: Red meat allergic patients
have a selective IgE response to the α-Gal glycan.
Allergy: European Journal of Allergy and Clinical Immunology 2015,
70(11):1497-1500.
- Mihajlovic L, Radosavljevic J, Burazer L, Smiljanic K, Cirkovic
Velickovic T: Composition of polyphenol and polyamide compounds in
common ragweed (Ambrosia artemisiifolia L.) pollen and sub-pollen
particles. Phytochemistry 2015,
109:125-132.
- Krstic M, Stojadinovic M, Smiljanic K, Stanic-Vucinic D, Cirkovic
Velickovic T: The anti-cancer activity of green tea, coffee and
cocoa extracts on human cervical adenocarcinoma HeLa cells depends on both
pro-oxidant and anti-proliferative activities of polyphenols.
RSC Advances 2015, 5(5):3260-3268.
- Apostolovic D, Stanic-Vucinic D, De Jongh HHJ, De Jong GAH, Mihailovic
J, Radosavljevic J, Radibratovic M, Nordlee JA, Baumert JL, Milcic M et al:
Conformational stability of digestion-resistant peptides of peanut
conglutins reveals the molecular basis of their allergenicity.
Scientific Reports 2016, 6.
- Apostolovic D, Krstic M, Mihailovic J, Starkhammar M, Velickovic TC,
Hamsten C, van Hage M: Peptidomics of an in vitro digested
α-Gal carrying protein revealed IgE-reactive peptides.
Scientific Reports 2017, 7.
- Minic SL, Stanic-Vucinic D, Mihailovic J, Krstic M, Nikolic MR,
Cirkovic Velickovic T: Digestion by pepsin releases biologically
active chromopeptides from C-phycocyanin, a blue-colored biliprotein of
microalga Spirulina. Journal of Proteomics 2016,
147:132-139.
- Perusko M, Al-Hanish A, Mihailovic J, Minic S, Trifunovic S, Prodic I,
Velickovic TC: Antioxidative capacity and binding affinity of the
complex of green tea catechin and beta-lactoglobulin glycated by the
Maillard reaction. Food Chemistry 2017,
232:744-752.
- Radibratovic M, Minic S, Stanic-Vucinic D, Nikolic M, Milcic M,
Velickovic TC: Stabilization of human serum albumin by the binding
of phycocyanobilin, a bioactive chromophore of blue-green alga spirulina:
Molecular dynamics and experimental study. PLoS ONE 2016,
11(12).
- Al-Hanish A, Stanic-Vucinic D, Mihailovic J, Prodic I, Minic S,
Stojadinovic M, Radibratovic M, Milcic M, Cirkovic Velickovic T:
Noncovalent interactions of bovine α-lactalbumin with green
tea polyphenol, epigalocatechin-3-gallate. Food
Hydrocolloids 2016, 61:241-250.
- Vesic J, Stambolic I, Apostolovic D, Milcic M, Stanic-Vucinic D,
Cirkovic Velickovic T: Complexes of green tea polyphenol,
epigalocatechin-3-gallate, and 2S albumins of peanut. Food
Chemistry 2015, 185:309-317.
- Minic SL, Milcic M, Stanic-Vucinic D, Radibratovic M, Sotiroudis TG,
Nikolic MR, Velickovic TC: Phycocyanobilin, a bioactive
tetrapyrrolic compound of blue-green alga Spirulina, binds with high
affinity and competes with bilirubin for binding on human serum
albumin. RSC Advances 2015,
5(76):61787-61798.
- Ognjenovic J, Stojadinovic M, Milcic M, Apostolovic D, Vesic J,
Stambolic I, Atanaskovic-Markovic M, Simonovic M, Velickovic TC:
Interactions of epigallo-catechin 3-gallate and ovalbumin, the
major allergen of egg white. Food Chemistry 2014,
164:36-43.
- Stojadinovic M, Radosavljevic J, Ognjenovic J, Vesic J, Prodic I,
Stanic-Vucinic D, Cirkovic Velickovic T: Binding affinity between
dietary polyphenols and β-lactoglobulin negatively correlates with the
protein susceptibility to digestion and total antioxidant activity of
complexes formed. Food Chemistry 2013,
136(3-4):1263-1271.
Članovi tima:
- dr Tanja Ćirković Veličković, redovni profesor biohemije
Univerziteta u Beogradu i redovni profesor hemije hrane Univerziteta u
Gentu
- dr Dragana Stanić-Vučinić, naučni savetnik
- dr Katarina Smiljanić, naučni saradnik
- dr Jelena Radosavljević - na postdoktorskom usavršavanju u
Institutu za hemiju i biohemiju Češke Akademije nauka u Republici Češkoj
- dr Marija Stojadinović - na postdoktorskom usavršavanju na
Institutu za infektivne bolesti u SAD
- dr Danijela Apostolović - na postdoktorskom usavršavanju na
Karolinska Institutu u Švedskoj
- dr Simeon Minić
- Jelena Vesić
- Milica Radibratović
- Maja Krstić
- Marija Peruško
- Ivana Prodić
- Mirjana Radomirović
- Smina Mukhtar
- Nina Savković
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