Academic year: |
2024/2025. |
Attendance requirements: |
There are no requirements. |
ECTS: |
8 |
Study level: |
graduate academic studies |
Study program: |
Biochemistry: 1. year, winter semester, compulsory course |
Teacher: |
Tanja D. Ćirković Veličković, Ph.D.
full professor, Faculty of Chemistry, Studentski trg 12-16, Beograd |
Assistant: |
Karla ®. Milčić, Ph.D.
assistant professor, Faculty of Chemistry, Studentski trg 12-16, Beograd |
Hours of instruction: |
Weekly: three hours of lectures + five hours of labwork (3+0+5) |
Course activities and grading method |
Lectures: |
10 points (3 hours a week)
Syllabus:
- An introduction to molecular biotechnology. Principles and applications of recombinant DNA Molecular biotechnology biological systems (procariotic and eukariotic) Eukariotic cells in culture DNA, RNA and protein synthesis.
- An introduction to molecular biotechnology. An overview of standard techniques applied in molecular biotechnology - including chromatography and electrophoresis, cloning techniques, gene expression systems, immunological methods, labeling of proteins and in situ-techniques, microscopy and laser systems.
- Cloning strategies Restriction endonucleases Plasmid cloning vectors Creating and screening a library Transformation of procariots cDNA libraries.
- Chemical synthesis and sequencing of DNA Amplification of DNA Sequencing of DNA.
- Gene manipulation strategies (protein engineering) Rational design of proteins Site-directed mutagenesis Dirigated evolution of proteins.
- Heterologous expression of proteins in procariotic systems.
- Manipulation of gens in procariots and optimization of gene expression in procariots.
- Heterologous expression of proteins in eucariotic systems.
- Saccharomyces cerevisiae expression system Other yeast expression systems Insect cells expression systems.
- Molecular biotechnology of microbial systems.
- Molecular diagnostics Microbial production of therapeutic agents.
- Vaccines.
- Synthesis of commercial products by recombinant microorganisms Restriction endonucleases Antibiotics Biopolymers Other examples.
- Microbial insecticides.
- Genetic engineering of plants: methodology and applications.
- Animal biotechnology.
- Transgenic animals.
- Regulating and patenting molecular biotechnology Ethical concerns in molecular biotechnology Patenting molecular biotechnology Legal, social and ethical aspects of biotechnology.
|
Labwork: |
10 points (5 hours a week) |
Colloquia: |
20 points |
Written exam: |
60 points |