University of Belgrade - Faculty of Chemistry Srpski - cirilica ЋИР   Srpski - latinica LAT

Home Home page   Map Site map   Links Links       Portals:   Employees For employees   Students For students   WebMail WebMail

About the Faculty
Departments and Centers
Studies
How to Become a Student
News
Museum of Chemistry
Student Organizations
Internal Pages

Pretraga Search the site:

[Project Logo]

Development of the assays for detection of SARS-CoV-2 virus capsid proteins in biological fluids of COVID-19 patients

Project acronym:   CAPSIDO

Project type:   COVID-19

Unique project identifier:   7542203

[Facebook]   [Twitter]
@CapsidoP

Project is funded by:   Science Fund of the Republic of Serbia (Belgrade, Serbia)

Instituions where the research is conducted:

Project realization is scheduled from December 22, 2020 till December 22, 2022.

Principal investigator:   Tanja D. Ćirković Veličković, University of Belgrade - Faculty of Chemistry (Beograd, Serbia)
      (click to display the list of investigators)

Repository for research results:   https://cherry.chem.bg.ac.rs/handle/123456789/4330

Short description of the project

The accurate diagnosis of people infected with the SARS-CoV-2 is essential to curb the global spread of COVID-19. The presence of SARS-CoV-2 can be detected by RT-PCR or by the presence of viral antigens in biological fluids using specific antibodies. Novel quantitative methods of detection of SARS-CoV-2 nucleocapsid (N) protein in biological fluids will be developed. Large scale recombinant production of N-protein and immunochemical methods (capture ELISA, immune-PCR test, immunoaffinity-based test) will be used for detection of N-protein in biological fluids. High resolution mass spectrometry-based methods will be used in immunoaffinity method development and recombinant protein characterization. Antibody-based tests for detection of SARS-CoV-2 antigens could provide more reliable and timely SARS-CoV-2 diagnosis of acute infection. ELISA is more convenient and economical than RTPCR, and serve as an alternative tool for the early diagnosis of SARS CoV-2 infection in laboratories and for mass screening for the reservoir of virus thus enabling better response to COVID19 pandemic.

[Graphical project abstract 1]
Capsido graphical abstract

Representative references

1. Teodora Djukic, Maja Mladenovic, Dragana Stanic-Vucinic, Jelena Radosavljevic, Katarina Smiljanic, Ljiljana Sabljic, Marija Devic, Danica Cujic, Tamara Vasovic, Ana Simovic, Mirjana Radomirovic, Tanja Cirkovic Velickovic. Expression, purification and immunological characterization of recombinant nucleocapsid protein fragment from SARS-CoV-2. Virology,2021,557:15-22,ISSN 0042-6822, https://doi.org/10.1016/j.virol.2021.01.004.(https://www.sciencedirect.com/science/article/pii/S004268222100012X)

Tue Jun 8 11:21:07 2021

© University of Belgrade - Faculty of Chemistry